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Frequently asked questions
General
Material
Analysis strategy
The deliverables provided by Argaly are:
• An analyses report with the different phases of the project (Methods, Results)
• A contingency table listing the identified taxa and the number of sequences associated for each sample
• A statistical and/or ecological analyses report if the study requires it
We provide an adapted quote for each new analysis request.
On a indicative basis, here are some global examples :
• Fish diversity analysis from 20 water samples : 250€ / sample.
• Global biodiversity analysis from 20 soil samples (Eukaryota/Bacteria) : 270€ / sample.
• Species detection analysis through qPCR : 105€ / sample.
We deliver the results of an eDNA study within 2-3 months after reception of the samples.
1. Low cost analysis and fast results
2. Higher detection reliability compared to traditional inventory methods
3. Easy sampling protocols
4. Field taxonomic expertise is not necessary
5. Inventory of multiple taxonomic groups in one single analysis
1. No information on individuals (age, sex, development stage...) and population size
2. Semi-quantitative information (for now...)
3. Taxonomic resolution depends on reference databases and primers
4. PCR and sequencing bias (errors, preferential amplifications...)
Semi-quantitative results are obtained through metabarcoding analyses.
The number of identified sequenced for each taxon is only comparable within one sample, thus the relative abondance of taxa can be represented to compare the analyzed samples.
If your samples correspond to already extracted DNA, please send us a minimum of 50µL per sample in order to perform all the experimental steps.
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