top of page

Frequently asked questions

  • What are the deliverables of an eDNA analysis?
    The deliverables provided by Argaly are: An analyses report with the different phases of the project (Methods, Results) A contingency table listing the identified taxa and the number of sequences associated for each sample A statistical and/or ecological analyses report if the study requires it
  • How much do biodiversity analyses based on environmental DNA cost?
    We provide an adapted quote for each new analysis request. On a indicative basis, here are some global examples : Fish diversity analysis from 20 water samples : 250€ / sample. Global biodiversity analysis from 20 soil samples (Eukaryota/Bacteria) : 270€ / sample. Species detection analysis through qPCR : 105€ / sample.
  • What is the period to get eDNA analysis results?
    We deliver the results of an eDNA study within 2-3 months after reception of the samples.
  • What are the advantages of the eDNA analysis methodology?
    Low cost analysis and fast results Higher detection reliability compared to traditional inventory methods Easy sampling protocols Field taxonomic expertise is not necessary Inventory of multiple taxonomic groups in one single analysis
  • What are the limits of the eDNA analysis method?
    No information on individuals (age, sex, development stage...) and population size Semi-quantitative information (for now...) Taxonomic resolution depends on reference databases and primers PCR and sequencing bias (errors, preferential amplifications...)
  • Are metabarcoding results quantitative?
    Semi-quantitative results are obtained through metabarcoding analyses. The number of identified sequenced for each taxon is only comparable within one sample, thus the relative abondance of taxa can be represented to compare the analyzed samples.
  • What volume of a DNA extract do I need to send for an eDNA analysis?
    If your samples correspond to already extracted DNA, please send us a minimum of 50µL per sample in order to perform all the experimental steps.
  • Is the sampling material sterile?
    Our sampling material is not certified "DNA free" but it is manufactured and prepared as sterilely as possible, avoiding external contaminations. Contaminations can be controlled by carrying out "field blanks" (for example, filtration of mineral water in the field for water samples or an empty vial left open during sampling for soil sampling).
  • What to do if any problem happens with the sampling material?
    Contact Céline Modugno on the following address: celine.modugno@argaly.com or by phone: +33748121252.
  • Can the sampling material be sent out of France?
    Yes, we send our material internationally.
  • Can aquatic sampling material undergo degradations when used in marine environments?
    Indeed, some parts of the filtration pump can be corroded following extended exposure to salty environments. It is thus recommended to store the filtration material out of boats when sampling is over.
  • What is the working duration of the pump?
    The pump can be used up to 12 hours until recharging.
  • What is the time persistance of DNA in the environment?
    Environmental DNA persistance depends on climatic and physicochemical conditions. In aquatic environments, DNA can persist between a few days and several weeks. In terrestrial environments, DNA persists from a few weeks up to several years!
  • What is the persistence distance of DNA in the environment?
    Environmental DNA can be transported passively or actively (deposit, percolation, flow, dissemination, movement of living organisms...). In aquatic environments, eDNA can be detected between several meters up to few kilometers from its original location. eDNA transport in terrestrial environments is much more limited, with a principal influence of organisms living under the ground surface.
  • How to determine an optimal sampling strategy for an eDNA analysis?
    An optimal sampling protocol for an eDNA analysis depends on the target organisms, the scientific question (global diversity VS specific species detection...) and statistical, logistical and financial constraints. Ideally, a sampling strategy maximizes the biological signal while minimizing the sampling effort. The collected samples must be the most representative of the studied ecosystem ; this implies the preliminary determination of the different habitats and biological replicates to be performed. Moreover, the area to sample and the sampling resolution are key factors to take into account. The sampling can be systematic; when environmental gradients exist or when the objective is to analyze distance influence; or randomized. In the case of an heterogenous environment, discretisation in unity and a stratified sampling can allow to increase the
bottom of page