eDNA filtration capsules

In partnership with the Canadian company Waterra, specialised in groundwater monitoring and sampling equipment, Argaly is a European reseller of eDNA filtration capsules. Those capsules are effective at recovering DNA from aquatic organisms from filtrations of large volumes of running or stagnant waters.

Technical caracteristics of the capsules

The commercialised capsules contain 600 cm² of high quality polyethersulphone 0.45 micron filter media
allowing to capture DNA from aquatic organisms.
Those filters can be used in high turbidity waters and filtration volumes can go up to 100 liters.
Other pore sizes are available on demand: 0.1, 0.2, 0.45, 1 and 5 micron. 

The filtration technique

The filtration technique proposed by Argaly, with a pumping system downstream of the capsule, eliminates the need to change the pipes between samplings and limits the risk of contamination.
One end of the pipe is placed on the capsule outlet and the other end is connected to the peristaltic pump. A second pipe placed on the pump outlet  allows the filtered water to be evacuated. 
As an example, a filtration of 30 minutes in clear water allows to collect around 40 liters of water (depending on the pump used).
Once filtration is complete, the capsule is emptied and then filled with DNA preservation buffer. Airtight caps are used to seal the capsule. After shaking the capsule, the preservation buffer will either be collected in a Falcon tube or stored in the capsule at room temperature until it is sent to the laboratory.

References

These filtration capsules have been compared with other methods for detecting aquatic species and their effectiveness has been demonstrated (see the scientific article on this subject: Pexoto et al. 2020).
Researchers from the Laboratory of Alpine Ecology of Grenoble (Dr Pierre Taberlet and Dr Fredreric Boyer) have also validated the use of those capsules in their eDNA research project on lagoon environments. 
To know more about the capsules, don't hesitate to contact us!